• Flexible, easy-to-follow workflow • High yield resulting in up to 70% time saving • Optimum workflow, minimal change of centrifugation speed, time, and pipetting volume • Allows researchers to correlate data generated from the same sample • High quality
Procedure without lysis step: 45 to 55 min. Procedure with lysis step: 50 to 85 min
2-D Protein Extraction Buffer for gel analysis: a convenient way to prepare high quality protein lysates.
• Allows high quality results for downstream applications such as 2-D gel analysis • Can also be used prior to 1-D electrophoresis, or for rehydrating IPG strips prior to 2-D electrophoresis • Supplied as a dry powder which is rehydrated with the provided diluent • Trial Kit allows you to evaluate each extraction buffer to find the most suitable buffer for your sample
Grinding kit: for disrupting small tissue and cell samples. Uses 1.5 ml microcentrifuge tubes, grinding resin and disposable pestles.
High-Abundant Protein Depletion: high-Abundant Protein Depletion kits conveniently remove >95% HSA and >90% IgG from 10 to 15 µl human serum, prior to 2-D electrophoresis
Mammalian and Yeast protein extraction buffer: for efficient and gentle extraction of biologically active, total soluble proteins from mammalian and yeast cultured cells
• Replaces laborious methods • High reproductibility • Total extraction of soluble proteins • Yeast buffer: suitable for preparation of approximately 10 ml yeast cell pellets suspension as single or as multiple smaller preps • Mammalian buffer: suitable for preparation of approximatively 10 ml of cell pellets
• Ready-to-use buffers for total protein extraction from tissues and cell lines, for 2-D gel analysis • Efficient and reproducible protein extraction with high yield • 6 different buffers covering the wide range of protein diversity