• Suitable for applications that require a lower charge • Designed for Southern and Northern blotting, colony and plaque lifts and Dot-/Slot-Blots • Compatible with isotopic and nonisotopic detection methods • Excellent signal-to-noise ratios • Moderate positive charge • Consistent membrane morphology • High sensitivity • Nytran binding capacity: >400 µg/ cm² • It is available in 0.2 µm and 0.45 µm pore sizes for optimal retention of oligos and larger DNA fragments
• Very high positive charge, low background • Nytran SuperCharge membrane binds nine times more molecules than a conventional nylon membrane • The great nylon density (compared to typical nylon membranes) provides increased binding sites for your samples • Excellent sy mmetry: gives the membrane the ability to lie flat • Very uniform pore size and pore distribution compared to typical nylon membranes: lead to greater reproducibility of results across a membrane and from blot to blot
Hybond-N • Strong, supported membrane • For nucleic acids blotting • Binding capacity up to 600 µg/ cm² • Recommended for radioactive blotting • Nucleic acids can be crosslinked to nylon using UV ligh
Hybond-N+ • For nucleic acids blotting • Binding capacity up to 600 µg/ cm² • For use with radioactive or nonradioactive chemiluminescence and chemifluorescence detection systems
Hybond-NX • For nucleic acids blotting • Binding capacity up to 600 µg/ cm² • Developed specifically for use with low hybridization buffer volumes and high-throughput applications • Gives clean backgrounds
Hybond-XL • For nucleic acids blotting • Binding capacity up to 600 µg/ cm² • Efficient binding of small fragments • Positively charged nylon membrane which is designed to give optimum signal-to-noise ratios when used with radioactively labeled probes
• Ready-to-use solution for blotting applications • Contains precut Amersham Hybond P 0.2 µm PVDF membranes preassembled with 2 x 3 mm Chr Blotting papers • The orientation of the sandwich must be so that the membrane is on the anode side of the gel • 3 mm Chr paper sheets cover each side of the sandwich • The gel is ready for transfer
• 100% pure nitrocellulose membrane 0.1 µm for molecules<7 kD • High binding, low background • High retention of small proteins: • 0.2 µm membrane retains samples of less than 20 KD • 0.45 µm membrane is designed for molecules over 20 KD • Excellent stability of proteins on the Protran (5 years) • No pre-wetting step with methanol: before the transfer, a simple wetting with water is enough • Excellent stability of the membrane: allows many manipulations
• For Western blotting • Optimised for use in conjunction with Amersham ECL detection reagents • Antibodies to IgG conjugated to horseradish peroxidase • Classical form (full antibody) or F(ab')2 fragment • F(ab')2 fragment form eliminates aspecific interactions of the Fc moiety, facilitates cell penetration, no interference with anti-Fc antibodies
The system is based on the direct labeling of DNA or RNA probes with thermostable alkaline phosphatase. The labeled probes are then hybridized with the target.
• Allows fluorescence detection of Proteins directly after SDS-PAGE or transfer • Excitation and emission wavelength:: 650 nm / 670 nm • Up to 150 samples • qualitative or quantitative marking according to staining time • Does not require discolouration step • Compatible with pre-cast gels • Compatible with chemiluminescence or fluorescent secondary marking • Without epitopes saturation for western blotting • Low background noise