Western Blot
Prepare →
Separate and blot →
Antibody incubation →
Detect ✓
Blotting Paper for Western Blotting
Azure Biosystems - AC2110-AC2111 Blotting Paper
Pre-cut blotting paper for Western blot transfer
Avoid measuring and cutting blotting paper to the size of your mini-gels. These pre-cut papers completely cover your gels and membranes.
- 8x10cm gels – blotting papers are 9x13cm
- 7x9cm gels – blotting papers are 8x10cm
Transfer Buffer
Advansta - FLASHBlot Transfer Buffer
Shorten your experimental time considerably
Designed for faster, more efficient protein transfer with increased sensitivity. The FLASHBlot transfer buffer will shorten your experimental time considerably.
- Faster
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More sensitive for low abundance proteins
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Better detection of post-translationally modified proteins
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Better transfer
Blotting Paper
Advansta - Blotting Paper Sheets
Pre-cut paper for electrophoretic transfers
This blotting paper is ideal for use in transfer sandwiches and cassettes with electrophoretic transfer.
- Works with most mini gel transfer assemblies
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Available in 7x9 and 8x10 cm
Protein Sample Loading Buffers
BioVision - 3X SDS-PAGE Loading Buffer
Ready-to-use buffer
BioVision's 3X SDS-PAGE Loading Buffer is a ready-to-use buffer solution for preparation of protein samples in SDS-PAGE. The buffer is also called Laemmli sample buffer.
- 10 mL (5 x 2 mL)
- Stored at -20 C°
Protein Sample Loading Buffers
Advansta - Protein Sample Loading Buffer
Pre-mixed standard Laemmli loading buffers for protein electrophoresis
Choose from reducing or non-reducing buffers. Standardized buffers helps you obtain reproducible results.
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Pre-mixed - just add an equal volume to your protein sample (supplied as 2X)
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Contain Bromophenol blue to enable visualization of the dye front and monitoring of the progress of electrophoresis
Protein Sample Loading Buffers
Santa Cruz Biotechnology - Electrophoresis Sample Buffer, 2X
Ready-to-use electrophoresis sample buffer
Electrophoresis Sample Buffer, 2X is a ready-to-use reducing electrophoresis sample buffer solution with bromophenol blue for preparation of protein samples in SDS-PAGE. Bromophenol is included as a tracking dye to monitor the sample during the electrophoretic run.
- Ready-to-use
- With bromophenol blue
- Contains DTT and TCEP-HCl, which disulfide bonds to ensure proteins are fully denatured before loading to the gel
Protein Ladder
Santa Cruz Biotechnology - Cruz Marker™ Molecular Weight Standards and Prestained Molecular Weight Standards
Consistent visibility on the final western blot image
Ready-to-use in SDS-PAGE loading buffer. Can be loaded directly into an SDS-PAGE gel.
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Sufficient for 50 gels
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Ladder consists of 6 bands (132, 90, 55, 43, 34, and 23 kDa)
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Must be used in combination with Cruz Marker™ MW Tag detection antibodies
Membranes
Santa Cruz Biotechnology - Membranes
Membranes for your Western blot experiments
Santa Cruz Biotechnology offer both Nitrocellulose and PVDF membranes that are ideal for use in transfer sandwiches and cassettes with electrophoretic transfer.
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UltraCruz® Nitrocellulose Pure Transfer Membranes
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UltraCruz® PVDF Transfer Membrane
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Immobilon®-FL PVDF membrane
Protein Ladder
Biovision - BriteRuler™ Pre-stained Protein Ladder
Ready-to-use protein ladder
Ready-to use formulation in gel loading buffer, requires no heating, dilution, or addition of reducing agents.
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Red and green reference bands included in the ladder
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100 applications
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Volume: 250 uL
Gel Loading Tips
Santa Cruz - ExactaCruz® Round Gel Loading Tips
Excellent tips for loading samples on gels
Suitable for a wide variety of vertical-loading gel applications with a 0.57 mm O.D.
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83 mm long and a 5 μl graduation mark for quick volume check
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Pipette tips are certified
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RNase/DNase-free, human DNA, and PCR inhibitor-free and non-pyrogenic
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Available in racks or in bulk bags
Gel Loading Tips
Eppendorf - GELoader®
Simplify the loading of your samples into gel electrophoresis
Excellent for handling the smallest volume into polyacrylamide gels.
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Flexible, long and narrow tips
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GELoader tips and rack autoclavable
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Prevents gels from getting damaged
High-quality liquid handling tools for molecular biology research
Sorenson Bioscience - Tubes, plates, tips, etc.
High-quality liquid handling tools for molecular biology research
Pipette tips, PCR products, microcentrifuge tubes, and MultiFlex gel loading tips from leading manufacturer Sorenson Bioscience. A range of consumables of high quality.
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Consistent quality performance
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Low-binding surface technology
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Certificate of analysis for each product
